Diane Saunders at TSL
We used the AT1 and AT2 assembled transcripts (in the github repository at mixed_material/ashwellthorpe_AT1/assemblies/AT1_trinity.fasta and mixed_material/ashwellthorpe_AT2/assemblies/AT2_trinity.fasta), which were assembled from reads generated from RNA extracted from infected branch pith material.
We collated the partial sequences of MAT1-1-3 and MAT1-2-1 proteins that have been identified in H. pseudoalbicans and used these in a tblastn search against the AT1 and AT2 assembled transcripts (with default settings). Further details on the H. pseudoalbidus MAT locus can be found in Gross et al., 2012.
The tblastn search highlighted transcripts from both assemblies with similarity to the MAT1-2-1 gene and not MAT1-1-3. The output can be found in the github repository (mixed_material/ashwellthorpe_AT1/blast/AT1_tblastn_MAT_locus and mixed_material/ashwellthorpe_AT2/blast/AT2_tblastn_MAT_locus). This suggests that the isolates AT1 and AT2 from Ashwellthorpe lower wood are of the MAT1-2 mating type, which is different to the KW1 C. fraxinea isolate from Kenninghall wood, which is of mating type MAT1-1.
Gross A., Zaffarano P.L., Duo A., Grunig C.R. (2012) Reproductive mode and life cycle of the ash dieback pathogen Hymenoscyphus pseudoalbicans. Fungal Genetics and Biology 49, 977-986.