The Contributors

Georgios Koutsovoulos, Mark Blaxter
The University of Edinburgh

Adam Vivian-Smith & Ari Hietala
Skog og Landskap – Norwegian Forest and Landscape Institute
Bioforsk – Norwegian Institute for Agricultural and Environmental Research

Renaud Ioos & colleagues
Unité de Mycologie, Laboratoire de la Santé des Végétaux, Domaine de Pixérécourt, Malzéville, France

Summary

Genome assembly summary of 23 Strains of H. pseudoalbidus sequenced at The University of Edinburgh

Table 1: 23 strains of H. pseudoalbidus from Europe

Strain	Year	Country
2008-81-6	2008	NORWAY
2008-125/2	2008	NORWAY
2008-139/1	2008	NORWAY
2008-142/5	2008	NORWAY
2008-148/4	2008	NORWAY
2008-152/4	2008	NORWAY
2009-86/3	2009	NORWAY
2010-189/4	2010	NORWAY
2010-189/5	2010	NORWAY
2011-11/1	2011	NORWAY
2012-24/1	2012	NORWAY
2012-38/2/2	2012	NORWAY
2012-42/1/1	2012	NORWAY
CBS122191	?	AUSTRIA
CBS122503	2011	POLAND
CBS122504	2005	POLAND
CBS122505	2000	POLAND
CBS122507	2000	POLAND
FON-M-1	2009	FRANCE
GIR-M-2	2009	FRANCE
LAN-M-1	2009	FRANCE
MIG-M-1	2009	FRANCE
LSVM82	2008	FRANCE

Table 2: Summary of assembly stats

Strain	contigs (>500)	span of contigs (MB)	N50	GC%	diff. from K1	indels from K1	span of indels
2008-125/2	6267	53.5	24303	42.67	193829	37014	75714
2008-139/1	6041	53.5	24414	42.69	174930	32599	68413
2008-142/5	6138	53.5	25254	42.72	180469	32502	69266
2008-148/4	7560	51.8	18776	43.18	175814	32875	68400
2008-152/4	6736	52.8	22327	42.9	176916	34024	70682
2008-81/6	7387	51.6	19538	43.19	187222	36522	72017
2009-86/3	6160	53.5	25295	42.72	164775	32603	64923
2010-189/4	6348	53.3	23729	42.78	179776	33438	70108
2010-189/5	6331	53.4	24880	42.77	179106	33429	69312
2011-11/1	6118	53.4	25094	42.73	188201	37279	75145
2012-24/1	6317	53.3	24080	42.77	177522	33805	69614
2012-38/2/2	6094	53.5	24711	42.67	174888	33466	67026
2012-42/1/1	6516	53.1	23078	42.83	173764	32218	67008
CBS122191	6252	53.5	24172	42.71	181217	32820	69030
CBS122503	6700	53.2	22119	42.77	195226	35872	74082
CBS122504	6365	56.7	30182	41.76	191934	33400	68770
CBS122505	6422	53.5	23708	42.67	172436	32440	66584
CBS122507	6387	53.1	23038	42.79	180121	34599	70054
FON-M-1	6436	53.3	23037	42.73	179944	35230	70160
GIR-M-2	4770	51.9	34647	43.59	196420	37771	95254
LAN-M-1	6534	52.5	23965	43.15	168898	31707	64028
LSVM82	35509	77.3	19479	38.33	171274	34758	92767
MIG-M-1	6498	52.8	23204	42.91	200012	36898	78521

Reports and Raw data

Reports and presentations of complete assemblies are available at OADB github Assembly reports of European strains

And raw data was submitted to The European Nucleotide Archive (ENA) ERP006093
Further information of raw data and assembled genomes are available at Genome assemblies of European strains
and at http://www.ebi.ac.uk/ena/data/view/ERS480843-ERS480865

The Contributors

Mark McMullan, Matt Clark, Louisa Williamson, James Lipscombe, Rachel Piddock, Fiore Cugliandolo, Fiona Fraser, Tom Barker, Mario Caccamo
The Genome Analysis Centre, Norwich

Summary

Hymenoscyphus pseudoalbidus (Chalara fraxinea) were isolated from across Great Britain and sent to TGAC by the The Food and Environment Research Agency (FERA). FERA purified DNA from each isolate which was sent to TGAC, where it was QC’d, libraries constructed and finally sequenced on a HiSeq2500 (150bp) paired end run. The 20 isolates were sequenced over two lanes (20 isolates per lane) to an average of ~50x depth per isolate.
Following are the 20 isolates
FERA_cc086
FERA_cc087
FERA_cc088
FERA_cc095
FERA_cc096
FERA_cc097
FERA_cc099
FERA_cc101
FERA_cc105
FERA_cc106
FERA_cc107
FERA_cc108
FERA_cc111
FERA_cc113
FERA_cc118
FERA_cc119
FERA_cc121
FERA_cc133
FERA_cc134
FERA_cc139

and their sequence information is available at OADB github

Downstream analyses (genome assembly, phylogenies and selection analyses) have been done at TGAC (MM).
These analyses await data from European isolates sequenced in Edinburgh.