The contributors
Anne Edwards at JIC
The material
We used DNA from primordial fruit bodies growing on dead ash raciness collected from Ashwellthorpe Lower Wood on 10th February 2013 and incubated at room temperature on moist filter paper for 10 weeks. The ITS region of 18S rDNA was amplified using universal primers: 5’-TCCGTAGGTGAACCTGCGG-3’ and 5’-TCCTCCGCTTATTGATATGC-3’ and Chalara specific primers 5’-AGCTGGGGAAACCTGACTG-3’ and 5’-ACACCGCAAGGACCCTATC-3’
The analysis
The DNA sequences (in the github repository at this link and this link) were blasted against the NCBI nucleotide database.
The interpretation
The structures are the primordial fruit bodies of Hymenoscyphus pseudoalbidus
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