Anne Edwards at JIC
We used DNA from fruiting bodies growing on dead ash racines and amplified the ITS region of 18S rDNA using universal primers: 5’-TCCGTAGGTGAACCTGCGG-3’ and 5’-TCCTCCGCTTATTGATATGC-3’
The DNA sequences were blasted against the NCBI nucleotide database.
The tblastn search identified two types of fruiting body
1) 92% identity (683/745) with Typhula phacorrhiza 18S rRNA
2) 97% identity (486/501) with Lachnum pudibundum 18s rRNA
This is the first report of these two fungal organisms at Ashwellthorpe lower wood.