Darren Soanes at the University of Exeter
DNA sequences containing internal transcribed spacers (ITS) and 5.8S rRNA were obtained from Genbank. The Chalaria fraxinea sequence came from isolate LWF_LB (Accession number JX667707.1). Sequences were also obtained from Genbank of ITS / 5.8S rRNA from a number of filamentous ascomycetes, especially Leotiomycetes (sequences cited in Wang et al, 2006 – http://www.ncbi.nlm.nih.gov/pubmed/16837216). Sequences were aligned using Muscle and positions containing gaps removed from the alignment. PhyML was used to create the phylogenetic tree (100 bootstraps) using a GTR substitution model and eight gamma rate categories. Two trees were produced:
1: general filamentous ascomycetes:
2: Leotiomycetes, with Stagonospora and Cochliobolus as outgroup:
The trees show that Chalaria fraxinea lies within the Hymenoscyphus clade of the Leotiomycetes (with good branch support). The most closely related species is Hymenoscyphus scutula.